Round with round pieces of paper or skin (usually a diameter of 2 ~ 4cm), at a certain concentration of fungicide solution or rotation dipping process for some time, be attached to a certain amount of coating suspension of mold spores to develop the central base plate, and then at a temperature of (28 ±1) ℃, relative humidity ≥95% of the training environment for some time, observed a round disk or circle around the transparent skin of whether the zone of inhibition, or suppression of vernier caliper bacteria circle diameter. Inhibition zone method is only a qualitative or semi-quantitative method, and the size of the zone of inhibition by the fungicide in the agar plate diffusion capacity (and the nature of preservatives, fungicides dissolved in the solvent type, training base composition of the mildew in the skin in the distribution of culture conditions are related to) a great impact, and therefore reference is limited. However, this method also has simple, visually recognizable, intuitive and good, commonly used in the preliminary evaluation of the effect of mold mildew.
II. minimum inhibitory concentration (MIC) method
To test antifungal preparation for a series of concentration, then Remove the aseptic 1mL, adding sterilized petri dishes, and then further injection of 1mL of the culture dish for the test bacteria suspension (or the use of ring vaccination to take crossed for the test bacteria suspension was inoculated added preservatives, and the solidification of the agar plate), and finally to the culture dish into the quantitative 40 ℃ melting agar, mixing, coagulation group, the placing temperature (28 ±1) ℃, ≥95% relative humidity environment to foster a period of time (usually 3 ~ 5d), check and compare all the agents can be tested strains minimum inhibitory concentration. MIC method is a quantitative method, it better reflects the size of mildew virulence, to facilitate the effect of different fungicide comparison between the mold is the most commonly used expression of virulence. However, many factors affect the MIC values, such as the source of the tested strains, inoculum size, mold spores, mold spores suspension in the number of medium, the solvent dissolves mildew species, cultivation conditions, therefore, to compare different fungicides MIC should be carried out under the same conditions.
III. Wet Room suspension method
After fungicide treatment will block the skin (usually 2.5cm ×5.0cm long box), for the test with a sprayer will spray mold spore suspension block in the skin surface, and then hung in the constant temperature and humidity box [temperature (28 ±1) ℃, relative humidity ≥95%] culture 28d, and regularly observe the mold situation, the mildew from the skin of the block to determine the effect of mold mildew.
The tested strains generally Aspergillus, Aspergillus flavus, Penicillium citrinum, Penicillium and Trichoderma top. This measurement method is to simulate the natural environment of accelerated test, the specimen mold force to 0 or 1 for qualified, the other for the failure. These methods have advantages and disadvantages of the same kind of mildew, use different methods of evaluation, the results may be quite different, even using the same method, because of the time, place and test conditions are different, the results will there are differences. Therefore, these accelerated laboratory test methods, only a certain reference value, mildew real application performance, must also be tested through practical application.
